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KMID : 0358819870140020153
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Journal of Korean Society of Plastic and Reconstructive Surgeons
1987 Volume.14 No. 2 p.153 ~ p.166
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IMMUNOHISTOCHEMICAL STUDIES ON THE POPULATION OF HUMAN EPIDERMAL LANGERHANS CELLS USING MONOCLONAL OKT??AND HLA-DR ANTIBODIES
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Kim Soo-Shin
Cho Sa-Sun
Cha Chang-Yong
Kim Chin-Hwan
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Abstract
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Reactivites of two monoclonal antibodies, OKT?? and HLA-DR, with epidermal Langerhans cells were studied in 142 skin specimens obtained by biopsy or surgery from various regions of the body. A sensitive avidin-biotin-peroxidase complex(ABC)method was applied to the staining of epidermal sheet preparations and frozen section
The statistical analysis of numbers of Langerhans cells observed under light microscope were summarized as follows.
1. The mean numbers of OKT??-positive dendritic cells were 789.6 cells/mm²in the epidermal sheet preparations, and 15.2 cells/linear mm in frozen sections.
2. The mean numbers of HLA-DR-positive dendritic cells were 711.9 cells/mm²in the epidermal sheet preparations, and 13.0 cells/linear mm in frozen sections.
3. HLA-DR antibody significantly underestimated Langerhans cells reactive with OKT6 both in epidermal sheet preparations(90.1%), and in frozen sections.(85.5%)
4. The mean numbers of dendritic cells reactive both with OKT?? and with HLA-DR were significantly higher in the back and abdomen, while in the prepuce they were significantly lower than those of other regions.
5. No significant sex difference in numbers of OKT?? - and HLA-DR-positive cells was observed both in epidermal sheet preparations and in frozen sections.
6. The double staining techniques showed that the majority of HLA-DR-positive cells also stained with OKT??.
7. The findings suggest that both antibodies are useful and reliable markers for identification of Langerhans cells.
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